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Seven new metabolites of drostanolone heptanoate by using Beauveria bassiana , and Macrophomina phaseolina cell suspension cultures.

Zahid HussainAtia-Tul- WahabNusrat HussainShabbir Hussainnull Atta-Ur-RahmanMouhamat Iqbal Choudhary
Published in: RSC advances (2020)
The present study reports the biotransformation of an anabolic-androgenic steroid (AAS) drostanolone heptanoate (1) by using two microbial cultures, Beauveria bassiana , and Macrophomina phaseolina . Fermentation of 1 with B. bassiana yielded five new transformed products 2-6, while with M. phaseolina it afforded two new 7-8, and two known 9-10 metabolites. The main sites of hydroxylation in the steroidal skeleton of 1 were at C-5, C-7, C-11, C-14, C-15, and C-20, hydrolysis of the ester moiety at C-17, and reduction of the carbonyl group at C-3. The structures of the transformed products were determined by using mass, NMR, and other spectroscopic techniques.
Keyphrases
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