Functional characterization of two TolC in the resistance to drugs and metals and in the virulence of Riemerella anatipestifer .
Li HuangFang GuoXiao LiMengying WangDekang ZhuMingshu WangRen-Yong JiaShun ChenXinxin ZhaoShaqiu ZhangQun GaoQiao YangYing WuJuan HuangBin TianXumin OuDi SunSai MaoLing ZhangYanLing YuFriedrich GötzAn-Chun ChengQinyuan ChenPublished in: Applied and environmental microbiology (2023)
(RA) is a notorious duck pathogen, characterized by a multitude of serotypes that exhibit no cross-reaction with one another. Moreover, RA is resistant to various antibacterial agents. Consequently, understanding the mechanisms behind resistance and identifying potential targets for drug development have become pressing needs. In this study, we show that the two TolC proteins play a role in the resistance to different drugs and metals and in the virulence. The results suggest that TolCA has a wider range of efflux substrates than TolCB. Except for gentamicin, neither TolCA nor TolCB was involved in the efflux of the other tested antibiotics. Strikingly, TolCA but not TolCB enhanced the frequency of resistance-conferring mutations. Moreover, TolCA was involved in RA virulence. Given its conservation in RA, TolCA has potential as a drug target for the development of therapeutics against RA infections.
Keyphrases
- rheumatoid arthritis
- pseudomonas aeruginosa
- escherichia coli
- disease activity
- staphylococcus aureus
- human health
- ankylosing spondylitis
- antimicrobial resistance
- interstitial lung disease
- emergency department
- health risk
- cystic fibrosis
- systemic sclerosis
- candida albicans
- silver nanoparticles
- climate change
- anti inflammatory
- electronic health record