An HBT-Based Near-Infrared Fluorescent Probe for Colorimetric and Ratiometric Detection of Bisulfite and its Application in Living Cells.

In this paper, a new type of ratiometric fluorescent probe HBT-TCF with large emission shift (up to 200 nm) has been developed for sensing HSO3- based on Michael-type addition reaction. The probe could fast recognize of HSO3- within 3 min in PBS buffer solution. The detection limit of the probe for HSO3- is as low as 101 nM. Meanwhile, the sensing mechanism is confirmed by NMR, LCMS. In addition, the probe can be applied to detect HSO3- in living cells.