A new polysaccharide (AABP-2B) was obtained from Anemarrhena asphodeloides Bunge after purification by gradient alcohol precipitation and DEAE-52 cellulose column chromatography. AABP-2B was confirmed to be a homogeneous polysaccharide with a molecular weight of 5800 Da and was composed of mannose and glucose at a molar ratio of 7.2 : 2.8. Structural analysis demonstrated that the backbone of AABP-2B was mainly composed of 4)-β-D-Man p -(1, 4,6)-β-D-Glc p -(1 and 3,6)-β-D-Man p -(1. The hypoglycaemic effect of AABP-2B was evaluated by its inhibition of α-glucosidase activities and insulin resistance in a HepG2 cell model. The results showed that AABP-2B displayed α-glucosidase inhibitory activities and could significantly improve glucose consumption by activating the IRS-1/PI3K/Akt signalling pathway in insulin-resistant HepG2 cells. Hence, AABP-2B may have potential as a functional food or medicine for diabetes therapy.
Keyphrases
- pi k akt
- signaling pathway
- type diabetes
- glycemic control
- blood glucose
- molecular docking
- cell cycle arrest
- liquid chromatography
- cell proliferation
- mass spectrometry
- single cell
- human health
- cell therapy
- cardiovascular disease
- high speed
- tandem mass spectrometry
- high performance liquid chromatography
- ionic liquid
- weight loss
- solid phase extraction
- blood pressure
- skeletal muscle
- smoking cessation
- climate change
- metabolic syndrome
- high resolution
- mesenchymal stem cells