Length-Dependent Cellular Internalization of Nanobody-Functionalized Poly(2-oxazoline) Nanorods.
John R FinneganLaura I FitzGeraldMoore Zhe ChenNicole M WarneDaniel YuenThomas P DavisAngus P R JohnstonKristian KempePublished in: Nano letters (2023)
The ability to target specific tissues and to be internalized by cells is critical for successful nanoparticle-based targeted drug delivery. Here, we combined "stealthy" rod-shaped poly(2-oxazoline) (POx) nanoparticles of different lengths with a cancer marker targeting nanobody and a fluorescent cell internalization sensor via a heat-induced living crystallization-driven self-assembly (CDSA) strategy. A significant increase in association and uptake driven by nanobody-receptor interactions was observed alongside nanorod-length-dependent kinetics. Importantly, the incorporation of the internalization sensor allowed for quantitative differentiation between cell surface association and internalization of the targeted nanorods, revealing unprecedented length-dependent cellular interactions of CDSA nanorods. This study highlights the modularity and versatility of the heat-induced CDSA process and further demonstrates the potential of POx nanorods as a modular nanomedicine platform.
Keyphrases
- cancer therapy
- drug delivery
- reduced graphene oxide
- cell surface
- high glucose
- diabetic rats
- induced apoptosis
- quantum dots
- drug induced
- papillary thyroid
- heat stress
- gene expression
- endothelial cells
- cell therapy
- young adults
- high throughput
- high resolution
- oxidative stress
- gold nanoparticles
- risk assessment
- cell cycle arrest
- climate change
- endoplasmic reticulum stress
- childhood cancer
- single molecule