IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma.
Annika BergauerNina SopelBettina KroßTytti VuorinenParaskevi XepapadakiScott T WeissAshley BlauHimanshu SharmaCornelia KrausRebekka SpringelManfred RauhSusanne MittlerAnna GraserTheodor ZimmermannVolker O MelicharAlexander KieferMarek L KowalskiAnna SobanskaTuomas JarttiHeikki LukkarinenNikolaos G PapadopoulosSusetta FinottoPublished in: The European respiratory journal (2017)
We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-α and IFN -: λ) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88, IRF1, STAT1 and STAT2 mRNA, whereas MYD88, IRF1, STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-α production was found increased, while IFN-λ secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV, asthmatic children can induce IFN-α secretion, indicating a hyperactive immune response to repeated respiratory virus infection.
Keyphrases
- dendritic cells
- mycobacterium tuberculosis
- chronic obstructive pulmonary disease
- immune response
- lung function
- young adults
- real time pcr
- emergency department
- toll like receptor
- physical activity
- intensive care unit
- escherichia coli
- transcription factor
- gene expression
- allergic rhinitis
- air pollution
- diabetic rats
- drug induced
- mass spectrometry
- zika virus
- sensitive detection
- copy number
- loop mediated isothermal amplification
- sleep quality
- aedes aegypti
- acute respiratory distress syndrome