Association of defects of enamel with polymorphisms in the vitamin D receptor and parathyroid hormone genes.
Amanda Renostro-SouzaGabriela Fonseca-SouzaErika Calvano KüchlerKatia Regina Felizardo VasconcelosJuliana Feltrin de SouzaChristian KirschneckMirian Aiko Nakane MatsumotoCesar Penazzo LepriMaria Angelica Hueb de Menezes OliveiraGeraldo Thedei JúniorPublished in: Brazilian dental journal (2024)
This cross-sectional study aimed to investigate the association between developmental defects of enamel (DDE) and single nucleotide polymorphisms (SNPs) in the genes encoding the vitamin D receptor (VDR) and parathyroid hormone (PTH). Orthodontic patients receiving treatment at a dental school were selected through convenience sampling. Intra-oral photographs were used to assess DDE, which were classified according to the criteria proposed by Ghanim et al. (2015) by a single calibrated examiner (Kappa>0.80). Enamel hypoplasia, molar-incisor hypomineralization (MIH), hypomimineralized second primary molar (HSPM), and non-MIH/HSPM demarcated opacities were considered for the analysis. Genomic DNA was extracted from buccal cells. The SNPs in VDR (rs7975232) and PHT (rs694, rs6256, and rs307247) were genotyped using real-time polymerase chain reactions (PCR). Statistical analyses were performed using the PLINK software (version 1.03, designed by Shaun Purcell, EUA). Chi-square or Fisher's exact tests were performed at a significance level of 5%. Ninety-one (n=91) patients (49 females and 42 males) (mean age of 14.1±5.8 years) were included. The frequency of DDE was 38.5% (35 patients). Genotype distributions were in Hardy-Weinberg equilibrium. No significant statistical association was found between DDE and the SNPs evaluated. A borderline association (p=0.09) was observed between DDE and the CC haplotype for SNP rs7975232 in VDR. In conclusion, the selected SNPs in VDR and PTH genes were not associated with DDE in the studied samples.
Keyphrases
- genome wide
- end stage renal disease
- ejection fraction
- newly diagnosed
- chronic kidney disease
- dna methylation
- peritoneal dialysis
- gene expression
- physical activity
- copy number
- oxidative stress
- induced apoptosis
- oral health
- cell free
- molecular dynamics
- signaling pathway
- psychometric properties
- genome wide analysis
- combination therapy