Antibacterial activity of paroxetine against Staphylococcus aureus and possible mechanisms of action.
Vitória Pessoa de Farias CabralDaniel Sampaio RodriguesAmanda Dias BarbosaLara Elloyse Almeida MoreiraLívia Gurgel do Amaral Valente SáCecília Rocha da SilvaJoão Batista de Andrade NetoJacilene SilvaEmanuelle Machado MarinhoHélcio Silva Dos SantosBruno Coêlho CavalcantiManoel O MoraesHélio Vitoriano Nobre JúniorPublished in: Future microbiology (2023)
Aim: To evaluate the antibacterial activity of paroxetine alone and associated with oxacillin against isolates of methicillin-sensitive and -resistant Staphylococcus aureus . Materials & methods: The broth microdilution and checkerboard techniques were used, with investigation of possible mechanisms of action through flow cytometry, fluorescence microscopy and molecular docking, in addition to scanning electron microscopy for morphological analysis. Results: Paroxetine showed a MIC of 64 μg/ml and bactericidal activity, mostly additive interactions in combination with oxacillin, evidence of action on genetic material and membrane, morphological changes in microbial cells and influence on virulence factors. Conclusion: Paroxetine has antibacterial potential from the perspective of drug repositioning.
Keyphrases
- staphylococcus aureus
- electron microscopy
- molecular docking
- flow cytometry
- biofilm formation
- silver nanoparticles
- single molecule
- methicillin resistant staphylococcus aureus
- high resolution
- induced apoptosis
- molecular dynamics simulations
- escherichia coli
- genome wide
- pseudomonas aeruginosa
- dna methylation
- risk assessment
- emergency department
- high speed
- high throughput
- oxidative stress
- genetic diversity
- climate change
- single cell
- human health
- gene expression
- drug induced
- cystic fibrosis