Towards the design of contrast-enhanced agents: systematic Ga 3+ doping on magnetite nanoparticles.
Itziar Galarreta-RodriguezLourdes MarcanoIdoia Castellanos-RubioIzaskun Gil de MuroIsabel GarcíaLuca OliviM L Fernández-GubiedaAinara Castellanos-RubioLuis LezamaIdoia Ruiz de LarramendiMaite InsaustiPublished in: Dalton transactions (Cambridge, England : 2003) (2022)
The main objective of the preparation of the Fe 3- x Ga x O 4 (0.14 ≤ x ≤ 1.35) system was to further the knowledge of the magnetic response of Ga 3+ -doped magnetite for application as MRI contrast agents. With this purpose, monodisperse nanoparticles between 7 and 10 nm with different amounts of gallium were prepared from an optimized protocol based on thermal decomposition of metallo-organic precursors. Thorough characterization of the sample was conducted in order to understand the influence of gallium doping on the structural, morphological and magnetic properties of the Fe 3- x Ga x O 4 system. X-ray diffraction and X-ray absorption near-edge structure measurements have proved the progressive incorporation of Ga in the spinel structure, with different occupations in both tetrahedral and octahedral sites. Magnetization measurements as a function of field temperature have shown a clear dependence of magnetic saturation on the gallium content, reaching an M s value of 110 Am 2 kg -1 at 5 K for x = 0.14 (significantly higher than bulk magnetite) and considerably decreasing for amounts above x = 0.57 of gallium. For this reason, nanoparticles with moderate Ga quantities were water-transferred by coating them with the amphiphilic polymer PMAO to further analyse their biomedical potential. Cytotoxicity assays have demonstrated that Fe 3- x Ga x O 4 @PMAO formulations with x ≤ 0.57, which are the ones with better magnetic response, are not toxic for cells. Finally, the effect of gallium doping on relaxivities has been analysed by measuring longitudinal (T 1 -1 ) and transverse (T 1 -1 ) proton relaxation rates at 1.4 T revealing that nanoparticles with x = 0.14 Ga 3+ content present remarkable T 2 contrast and the nanoparticles with x = 0.26 have great potential to act as dual T 1 -T 2 contrast agents.
Keyphrases
- pet ct
- contrast enhanced
- magnetic resonance
- magnetic resonance imaging
- molecularly imprinted
- diffusion weighted
- computed tomography
- healthcare
- diffusion weighted imaging
- dual energy
- cell proliferation
- induced apoptosis
- walled carbon nanotubes
- high throughput
- photodynamic therapy
- mass spectrometry
- endoplasmic reticulum stress
- cell death
- signaling pathway
- human health
- oxidative stress
- transition metal
- tandem mass spectrometry
- water soluble