Anti-Inflammatory Cembrane-Type Diterpenoids and Prostaglandins from Soft Coral Lobophytum sarcophytoides.
Hongjie ShenXiaowan LiuMinghua JiangGuangyuan LuoZhenger WuBin ChenJing LiLan LiuSen-Hua ChenPublished in: Marine drugs (2019)
Two new cembrane-type diterpenoids, lobophytins A (1) and B (3), and four new prostaglandins, (5E)-PGB2 (10), (5E)-13,14-dihydro-PGB2 (11), 13,14-dihydro-PGB2 (12) and 13,14-dihydro-PGB2-Me (13), together with ten known compounds were isolated from the soft coral Lobophytum sarcophytoides. The structures of these new secondary metabolites were identified by high resolution mass spectrometry (HR-ESIMS), nuclear magnetic resonance (NMR) and electron circular dichroism (ECD) analyses, as well as the modified Mosher's method. Compounds 6, 7, 9, 10, 12, 13, 15 and 16 showed potential anti-inflammatory activity by inhibiting the production of nitric oxide (NO) in RAW264.7 cells that were activated by lipopolysaccharide, with IC50 values ranging from 7.1 to 32.1 μM and were better than the positive control indomethacin, IC50 = 39.8 μM.
Keyphrases
- magnetic resonance
- high resolution mass spectrometry
- nitric oxide
- anti inflammatory
- induced apoptosis
- liquid chromatography
- high resolution
- signaling pathway
- ultra high performance liquid chromatography
- mass spectrometry
- gas chromatography
- inflammatory response
- toll like receptor
- cell cycle arrest
- lps induced
- endoplasmic reticulum stress
- contrast enhanced
- oxidative stress
- nitric oxide synthase
- immune response
- computed tomography
- human health