Glycosylation profiles of breast cancer cells may represent clonal variations of multiple organ metastases.
Yoshiya HorimotoMay Thinzar HlaingHarumi SaekiKaori Denda-NagaiKatrin Ishii-SchradeHaruhiko FujihiraMasaaki AbeMiki NojiShigeyuki ShichinoMitsue SaitoTatsuro IrimuraPublished in: Clinical & experimental metastasis (2024)
Glycosylation changes of cancer cells are known to be associated with malignant progression and metastases and potentially determine the organ-selective nature of metastasis as theorized by Paget (Lancet 1:571-573, 1889). Cellular glycans play a variety of roles in the processes of metastasis and may be unique to the cells that metastasize to different organs. We analyzed the glycosylation profiles of the primary tumor and tumors metastasized to lymph node, liver, lung, brain, bone, thyroid, kidney, adrenal, small intestine and pancreas in an autopsy case of breast cancer employing a lectin microarray with 45 lectins. Clustering analysis of the data revealed that metastatic breast cancer cells were categorized into several clusters according to their glycosylation profiles. Our results provide a biological basis to understand differential phenotypes of metastatic breast cancer cells potentially reflecting clonal origin, which does not directly reflect genomic or genetic changes or microenvironmental effects but connects to glycosylation profiles.
Keyphrases
- breast cancer cells
- lymph node
- squamous cell carcinoma
- small cell lung cancer
- induced apoptosis
- single cell
- copy number
- gene expression
- early stage
- genome wide
- radiation therapy
- machine learning
- resting state
- dna methylation
- signaling pathway
- big data
- functional connectivity
- cell proliferation
- neoadjuvant chemotherapy
- cell death
- cell cycle arrest
- rna seq
- brain injury
- body composition
- artificial intelligence
- bone loss
- bone regeneration
- locally advanced