A surrogate molecular approach for the detection of Philadelphia chromosome-like B-acute lymphoblastic leukemia.
Dikshat Gopal GuptaNeelam VarmaSarki Abba AbdulkadirSreejesh SreedharanunniMan Updesh Singh SachdevaShano NaseemParveen BoseJogeshwar BinotaPankaj MalhotraAlka KhadwalAmita TrehanSubhash VarmaPublished in: Cancer (2023)
B-acute lymphoblastic leukemia (B-ALL) cases using multiplex-reverse transcriptase polymerase chain reaction. Identification and characterization of Philadelphia (Ph)-like ALL cases using nCounter NanoString gene expression profiling and fluorescence in situ hybridization. Furthermore, Ph-like ALL cases were characterized according to CRLF2 expression and kinase-activating genomic alterations. Minimal residual disease of Ph-like ALL cases were quantified using flow cytometry-minimal residual disease assay. A surrogate molecular approach was established to detect Ph-like ALL cases from low- and middle-income countries.