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Rapid Identification of Lineage and Drug Resistance in Clinical Samples of Mycobacterium tuberculosis .

Jéssica ComínJesús ViñuelasCarmen LafozAlberto CebolladaDaniel IbarzMaría José IglesiasSofía Samper
Published in: Microorganisms (2023)
This technique has demonstrated high sensitivity in obtaining the drug-resistance profile of the isolates, as even those samples with DNA concentrations below the detection limit of Qubit produced a result. AmpliSeq technology is cheaper than whole-genome sequencing, easy to perform by laboratory technicians and applicable to any microorganism using the Ion Torrent platform.
Keyphrases
  • mycobacterium tuberculosis
  • loop mediated isothermal amplification
  • circulating tumor
  • high throughput
  • single molecule
  • single cell
  • label free
  • sensitive detection