Ultrasensitive Detection of SARS-CoV-2 Antibody by Graphene Field-Effect Transistors.
Hua KangXuejun WangMingquan GuoChanghao DaiRenzhong ChenLei YangYanling WuTianlei YingZhaoqin ZhuDapeng WeiYunqi LiuDacheng WeiPublished in: Nano letters (2021)
The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10-18 M (equivalent to 10-16 g mL-1) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of ∼150 antibodies in 100 μL full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.
Keyphrases
- sars cov
- label free
- loop mediated isothermal amplification
- respiratory syndrome coronavirus
- public health
- real time pcr
- gold nanoparticles
- quantum dots
- sensitive detection
- machine learning
- mass spectrometry
- carbon nanotubes
- deep learning
- walled carbon nanotubes
- simultaneous determination
- molecularly imprinted
- liquid chromatography