Increased acquired protease inhibitor drug resistance mutations in minor HIV-1 quasispecies from infected patients suspected of failing on national second-line therapy in South Africa.
Adetayo Emmanuel ObasaAnoop T AmbikanSoham GuptaUjjwal NeogiGraeme Brendon JacobsPublished in: BMC infectious diseases (2021)
HTS-GRT improved the identification of PI and reverse transcriptase inhibitor (RTI) RAMs in second-line cART patients from South Africa compared to the conventional GRT with ≥20% used in Sanger-based sequencing. Several RTI RAMs, such as K65R, M184V or K103N and PI RAM V82A, were identified in < 20% of the population. Deep sequencing could be of greater value in detecting acquired resistance mutations early.
Keyphrases
- south africa
- hiv positive
- end stage renal disease
- single cell
- ejection fraction
- antiretroviral therapy
- chronic kidney disease
- newly diagnosed
- hiv infected
- prognostic factors
- pulmonary embolism
- hiv aids
- peritoneal dialysis
- human immunodeficiency virus
- stem cells
- hiv testing
- patient reported outcomes
- patient reported
- smoking cessation