Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria.
Tian Fook KongXinhui ShenMarcos MarcosChun YangImran Halimi IbrahimPublished in: Biomicrofluidics (2020)
In this work, a dielectrophoretic impedance measurement (DEPIM) lab-on-chip device for bacteria trapping and detection of Escherichia coli, Vibrio cholerae, and Enterococcus is presented. Through the integration of SU-8 negative photoresist as a microchannel and the precise alignment of the SU-8 microchannel with the on-chip gold interdigitated microelectrodes, bacteria trapping efficiencies of up to 97.4%, 97.7%, and 37.7% were achieved for E. coli, V. cholerae, and Enterococcus, respectively. The DEPIM device enables a high detection sensitivity, which requires only a total number of 69 ± 33 E. coli cells, 9 ± 2 Vibrio cholera cells, and 36 ± 13 Enterococcus cells to observe a discernible change in system impedance for detection. Nonetheless, the corrected limit of detection for Enterococcus is 95 ± 34 after taking into consideration the lower trapping efficiency. In addition, a theoretical model is developed to allow for the direct estimation of the number of bacteria through a linear relationship with the change in the reciprocal of the overall system absolute impedance.
Keyphrases
- magnetic resonance imaging
- escherichia coli
- biofilm formation
- induced apoptosis
- contrast enhanced
- loop mediated isothermal amplification
- real time pcr
- label free
- staphylococcus aureus
- pseudomonas aeruginosa
- endoplasmic reticulum stress
- magnetic resonance
- signaling pathway
- cystic fibrosis
- single cell
- cell proliferation
- methicillin resistant staphylococcus aureus
- neural network