Resistome Profiles, Plasmid Typing, and Whole-Genome Phylogenetic Tree Analyses of BlaNDM-9 and Mcr-1 Co-Harboring Escherichia coli ST617 from a Patient without a History of Farm Exposure in Korea.
Le Phuong NguyenNaina Adren PintoThao Nguyen VuHung MaiAn Ht PhamHyunsook LeeYoung Lag ChoJung-Hyun ByunRoshan D'SouzaDongeun YongPublished in: Pathogens (Basel, Switzerland) (2019)
Recently, a blaNDM-9 and mcr-1 co-harboring E. coli ST 617 isolate was identified from an asymptomatic carrier in Korea. An 81-year-old female was admitted to a university hospital for aortic cardiac valve repair surgery. Following surgery, she was admitted to the intensive care unit (ICU) for three days, and carbapenem-resistant E. coli YMC/2017/02/MS631 was isolated from a surveillance culture (rectal swab). Antimicrobial susceptibility testing (AST) for colistin was not performed at that time. Upon retrospective study, further AST revealed resistance to all tested antibiotics, including meropenem, imipenem, ceftazidime-avibactam, amikacin, gentamicin, ciprofloxacin, trimethoprim-sulfamethoxazole, and colistin, with the exception of tigecycline. Whole genome sequencing analyses showed that this strain belonged to the ST617 serotype O89/162: H10 and harbored three β-lactamase genes (blaTEM-1B, blaCTX-M-55, blaNDM-9), mcr-1, and 14 other resistance genes. Seven plasmid replicon types (IncB, IncFII, IncI2, IncN, IncY, IncR, IncX1) were identified. Horizontal transfer of blaNDM-9 and mcr-1 from donor cells to the recipient E. coli J53 has been observed. blaNDM-9 and mcr-1 were carried by IncB and IncI2 plasmids, respectively. To speculate on the incidence of this strain, routine rectal swab screening to identify asymptomatic carriers might be warranted, in addition to the screening of ICU patients.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- minimally invasive
- biofilm formation
- end stage renal disease
- aortic valve
- coronary artery bypass
- intensive care unit
- multidrug resistant
- genome wide
- ejection fraction
- left ventricular
- public health
- gram negative
- chronic kidney disease
- newly diagnosed
- rectal cancer
- mass spectrometry
- multiple sclerosis
- prognostic factors
- mechanical ventilation
- case report
- mitral valve
- gene expression
- aortic stenosis
- ms ms
- heart failure
- dna methylation
- percutaneous coronary intervention
- cell death
- atrial fibrillation
- crispr cas
- acute coronary syndrome
- patient reported
- pulmonary arterial hypertension