Rapid cyclic ion mobility separations of monosaccharide building blocks as a first step toward a high-throughput reaction screening platform for carbohydrate syntheses.
Tyler L PetersonGabe NagyPublished in: RSC advances (2021)
Herein we present a new high-throughput screening method for carbohydrate syntheses based on cyclic ion mobility spectrometry-mass spectrometry (cIMS-MS)-based separations. We rapidly resolved the α/β anomers for carbohydrates with varying protecting groups after only 5 m of cIMS-MS separation and also detected their respective unwanted anomeric impurities at levels lower than 2%. All experiments were performed in 1 minute of total acquisition time demonstrating our method's high-throughput nature. Our methodology was also extended to the separation of an isomeric mixtures of two protected disaccharides illustrating its utility beyond only monosaccharides. We envision our presented workflow as a first step toward the development of a high-throughput screening platform for the rapid and sensitive detection of α/β anomeric selectivities and for trace isomeric/isobaric impurities.
Keyphrases
- high throughput
- mass spectrometry
- liquid chromatography
- sensitive detection
- loop mediated isothermal amplification
- capillary electrophoresis
- gas chromatography
- high resolution
- tandem mass spectrometry
- single cell
- solid phase extraction
- quantum dots
- high performance liquid chromatography
- multiple sclerosis
- ms ms
- simultaneous determination
- ionic liquid
- heavy metals
- electronic health record