Chaperone-substrate interactions monitored via a robust TEM-1 β-lactamase fragment complementation assay.
Ling BaiWei HeTianpeng LiCuiting YangYingping ZhuangShu QuanPublished in: Biotechnology letters (2017)
The β-lactamase PCA system faithfully reports chaperone-substrate interactions in the bacterial cell envelope, and therefore this system has the potential to map the complex protein homeostasis network under a fluctuating environment.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- heat shock protein
- gram negative
- multidrug resistant
- amino acid
- single cell
- endoplasmic reticulum
- heat shock
- high throughput
- cell therapy
- structural basis
- protein protein
- emergency department
- stem cells
- human health
- small molecule
- oxidative stress
- electronic health record
- heat stress
- network analysis