Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

Samuel A MyersJason WrightRyan PecknerBrian T KalishFeng ZhangSteven A Carr

Published in: Nature methods (2018)

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

Keyphrases

gene expression
genome wide
copy number
mass spectrometry
dna methylation
genome wide association study
small molecule
crispr cas
transcription factor
high throughput
label free
drug delivery
genome wide identification
single cell