Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid.
Galina B GusevaElena V AntinaMikhail B BerezinRoman S PavelyevAirat R KayumovOlga V OstolopovskayaIlmir R GilfanovLarisa L FrolovaAlexander V KutchinRustem F AkhverdievSvetlana A LisovskayaElena Y TriznaOlga A LodochnikovaDaut R IslamovSergey V EfimovVladimir V KlochkovIlya A KhodovSergei V BoichukLiliya E NikitinaPublished in: ACS applied bio materials (2021)
This article describes the design and biological properties of a BODIPY FL-labeled monoterpenoid BF 2 - meso -(4-((1″ R )-6″,6″-dimethylbicyclo[3.1.1]hept-2″-ene-2″)yl-methoxycarbonylpropyl)-3,3',5,5'-tetramethyl-2,2'-dipyrromethene conjugate (BODIPYmyrt). The fluorophore was characterized using X-ray, NMR, MS, and UV/vis spectroscopy. The conjugate exhibits a high quantum yield (to ∼100%) in the region 515-518 nm. BODIPYmyrt effectively penetrates the membranes of the bacterial and fungal cells and therefore can be used to examine the features of a broad spectrum of Gram-positive and Gram-negative bacteria and pathogenic fungi as well. Moreover, BODIPYmyrt exhibits a moderate tropism to the subcellular structures in mammalian cells (e.g., mitochondria), thereby providing an attractive scaffold for fluorophores to examine these particular organelles.
Keyphrases
- high resolution
- fluorescent probe
- living cells
- mass spectrometry
- induced apoptosis
- pet imaging
- cell cycle arrest
- solid state
- cancer therapy
- dual energy
- multiple sclerosis
- cell death
- magnetic resonance
- ms ms
- optical coherence tomography
- single molecule
- molecular dynamics
- gram negative
- signaling pathway
- endoplasmic reticulum stress
- computed tomography
- oxidative stress
- drug delivery
- cell proliferation
- energy transfer
- endoplasmic reticulum
- aqueous solution
- cell wall