Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin Against Daptomycin-Nonsusceptible Enterococcus faecium.
Taylor MorrisetteKatherine L LevGregory S CanfieldBreck A DuerkopRazieh KebriaeiKyle C StamperDana HolgerSusan M LehmanSmaranda WillcoxCesar A AriasMichael Joseph RybakPublished in: Antimicrobial agents and chemotherapy (2021)
Enterococcus faecium(E. fcm) is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. fcm strains against 4 phages, two phages(113, 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin(DAP) plus phage cocktail(113:myophage;9184:siphopage) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin non-susceptible E. fcm.
Keyphrases
- methicillin resistant staphylococcus aureus
- multidrug resistant
- electron microscopy
- pseudomonas aeruginosa
- biofilm formation
- staphylococcus aureus
- drug resistant
- candida albicans
- acinetobacter baumannii
- gram negative
- genome wide
- cystic fibrosis
- klebsiella pneumoniae
- bone marrow
- dna methylation
- mesenchymal stem cells