The Distribution of Eight Antimicrobial Resistance Genes in Streptococcus oralis , Streptococcus sanguinis , and Streptococcus gordonii Strains Isolated from Dental Plaque as Oral Commensals.
Verónica Morales-DorantesRubén Abraham Domínguez PérezRosa Martha Pérez-SerranoJuan Carlos Solís-SainzPablo García-SolísLeón Francisco Espinosa-CristobalClaudia Verónica Cabeza-CabreraJosé Luis Ayala-HerreraPublished in: Tropical medicine and infectious disease (2023)
It has been proposed that oral commensal bacteria are potential reservoirs of a wide variety of antimicrobial resistance genes (ARGs) and could be the source of pathogenic bacteria; however, there is scarce information regarding this. In this study, three common streptococci of the mitis group ( S. oralis , S. sanguinis , and S. gordonii ) isolated from dental plaque (DP) were screened to identify if they were frequent reservoirs of specific ARGs ( blaTEM , cfxA , tetM , tetW , tetQ , ermA , ermB , and ermC ). DP samples were collected from 80 adults; one part of the sample was cultured, and from the other part DNA was obtained for first screening of the three streptococci species and the ARGs of interest. Selected samples were plated and colonies were selected for molecular identification. Thirty identified species were screened for the presence of the ARGs. From those selected, all of the S. sanguinis and S. oralis carried at least three, while only 30% of S. gordonii strains carried three or more. The most prevalent were tetM in 73%, and blaTEM and tetW both in 66.6%. On the other hand, ermA and cfxA were not present. Oral streptococci from the mitis group could be considered frequent reservoirs of specifically tetM , blaTEM , and tetW . In contrast, these three species appear not to be reservoirs of ermA and cfxA .
Keyphrases
- antimicrobial resistance
- antibiotic resistance genes
- biofilm formation
- candida albicans
- bioinformatics analysis
- escherichia coli
- coronary artery disease
- genome wide
- wastewater treatment
- oral health
- microbial community
- magnetic resonance
- single molecule
- healthcare
- genome wide identification
- genetic diversity
- pseudomonas aeruginosa
- magnetic resonance imaging
- gene expression
- computed tomography
- cell free
- endothelial cells
- circulating tumor
- cystic fibrosis
- anaerobic digestion
- contrast enhanced
- risk assessment
- health information
- nucleic acid
- human health
- transcription factor
- climate change