Comparison of the dentin tubule penetration of AH Plus, WellRoot ST, and MTA BioSeal after obturation, retreatment, and re-shaping of the root canals.

It was aimed to compare the dentine tubule penetration of AH Plus, MTA Bioseal, and WellRoot ST after filling, retreating, and reshaping the root canals by using a novel microscopic method. Seventy-five mandibular incisors with single root canals were shaped with Protaper Next system (PTN; X2-25/0.06) The teeth were obturated with AH Plus labeled with Rhodamin B and MTA Bioseal or WellRoot ST sealer which were labeled with Fluo-3. Then the root canal fillings were removed with Protaper Universal Retreatment files. At the last stage, the teeth were reshaped with PTN (X3-30/0.07). The samples were transversally sectioned and examined using a Cytation 5 reader and Gen5 software regarding the mean and the maximum depth of sealer penetration. No significant difference was observed between the groups, except for the 6 mm level after reshaping the root canals (p < .05). It is not possible to completely remove the sealer remnants from the dentin walls even after reshaping the root canals. RESEARCH HIGHLIGHTS: Even after reshaping the root canals in the retreatment procedure, it is not possible to completely remove the filling residues of the epoxy-resin-based, calcium-silicate-based and MTA-based endodontic pastes from the dentin walls.