Discovery of (Dihydro)pyrazine N-Oxides via Genome Mining in Pseudomonas.
Ashley M KretschGina L MorganJillian TyrrellEmily MeversIsabelle Vallet-GélyBo LiPublished in: Organic letters (2018)
Overexpression of the Pseudomonas virulence factor ( pvf) biosynthetic operon led to the identification of a family of pyrazine N-oxides (PNOs), including a novel dihydropyrazine N,N'-dioxide (dPNO) metabolite. The nonribosomal peptide synthetase responsible for production of (d)PNOs was characterized, and a biosynthetic pathway for (d)PNOs was proposed. This work highlights the unique chemistry catalyzed by pvf-encoded enzymes and sets the stage for bioactivity studies of the metabolites produced by the virulence pathway.
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- escherichia coli
- staphylococcus aureus
- antimicrobial resistance
- candida albicans
- small molecule
- ms ms
- cell proliferation
- high throughput
- transcription factor
- cystic fibrosis
- genome wide
- plant growth
- room temperature
- dna methylation
- bioinformatics analysis
- single cell
- ionic liquid
- light emitting