Photo-Activated Ratiometric Fluorescent Indicator for Real-Time and Visual Detection of Plasma Membrane Homeostasis.
Yingcui BuHaoran WangYu DengFeng ZhongZhi-Peng YuXiaojiao ZhuHong-Ping ZhouPublished in: Analytical chemistry (2023)
Development of an activated ratiometric indicator that is specific to plasma membrane (PM) viscosity exhibits great application prospects in disease diagnosis and treatment but remains a great challenge. Herein, a photo-activated fluorescent probe ( CQ-IC ) was designed and prepared tactfully, which could analyze and real-time monitor the microenvironmental homeostasis of the PM based on a two-channel ratiometric imaging model. Interestingly, upon light irradiation, CQ-IC generates reactive oxygen species and thus increases the cellular viscosity, which increases two emission peaks at 480 and 610 nm. This work would propose a new strategy to sensor PM homeostasis and effectively guide the treatment of viscosity-related diseases among various physiological and pathological processes.
Keyphrases
- fluorescent probe
- living cells
- particulate matter
- air pollution
- reactive oxygen species
- polycyclic aromatic hydrocarbons
- heavy metals
- quantum dots
- water soluble
- high resolution
- single molecule
- photodynamic therapy
- label free
- current status
- electron transfer
- combination therapy
- nitric oxide
- replacement therapy
- fluorescence imaging