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Stereoselective amino acid synthesis by synergistic photoredox-pyridoxal radical biocatalysis.

Lei ChengDian LiBinh Khanh MaiZhiyu BoLida ChengPeng LiuYang Yang
Published in: Science (New York, N.Y.) (2023)
Developing synthetically useful enzymatic reactions that are not known in biochemistry and organic chemistry is an important challenge in biocatalysis. Through the synergistic merger of photoredox catalysis and pyridoxal 5'-phosphate (PLP) biocatalysis, we developed a pyridoxal radical biocatalysis approach to prepare valuable noncanonical amino acids, including those bearing a stereochemical dyad or triad, without the need for protecting groups. Using engineered PLP enzymes, either enantiomeric product could be produced in a biocatalyst-controlled fashion. Synergistic photoredox - pyridoxal radical biocatalysis represents a powerful platform with which to discover previously unknown catalytic reactions and to tame radical intermediates for asymmetric catalysis.
Keyphrases
  • amino acid
  • visible light
  • cancer therapy
  • high throughput
  • mass spectrometry
  • nitric oxide