Individual Genomic Loci and mRNA Levels of Immune Biomarkers Associated with Pneumonia Susceptibility in Baladi Goats.
Ahmed AteyaMona Al-SharifMohamed AbdoLiana FericeanBothaina EssaPublished in: Veterinary sciences (2023)
The effectiveness of breeding for inherent disease resistance in animals could be considerably increased by identifying the genes and mutations that cause diversity in disease resistance. One hundred and twenty adult female Baladi goats (sixty pneumonic and sixty apparently healthy) were used in this study. DNA and RNA were extracted from blood samples collected from the jugular vein of each goat. SLC11A1 , CD-14 , CCL2 , TLR1 , TLR7 , TLR8 , TLR9 , β defensin , SP110 , SPP1 , BP1 , A2M , ADORA3 , CARD15 , IRF3 , and SCART1 SNPs that have been previously found to be associated with pneumonia resistance/susceptibility were identified via PCR-DNA sequencing. The pneumonic and healthy goats differed significantly, according to a Chi-square analysis of the discovered SNPs. The mRNA levels of the studied immune markers were noticeably greater in the pneumonic goats than in the healthy ones. The findings could support the significance of the use of immune gene expression profiles and nucleotide variations as biomarkers for the susceptibility/resistance to pneumonia and provide a practical management technique for Baladi goats. These results also suggest a potential strategy for lowering pneumonia in goats by employing genetic markers linked to an animal's ability to fend off infection in selective breeding.
Keyphrases
- genome wide
- toll like receptor
- inflammatory response
- immune response
- copy number
- dna methylation
- single molecule
- nuclear factor
- systematic review
- community acquired pneumonia
- respiratory failure
- binding protein
- single cell
- dendritic cells
- genome wide identification
- genome wide association
- acute respiratory distress syndrome
- liver fibrosis
- extracorporeal membrane oxygenation
- mechanical ventilation
- real time pcr
- human health