Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex.

Abdullah AlbastakiMohammed NajiReem LootahReem AlmheiriHanan AlmullaAfra AlreyamiIman AlmarriZailiang YuYueke ZhangChang ShuRashed Alghafri

Published in: Electrophoresis (2022)

This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

Keyphrases

real time pcr
high throughput
magnetic resonance imaging
gene expression
computed tomography
risk assessment
single cell