Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.

Debbie Watson Sam R Adhikary Peter Cuthbertson Nicholas J Geraghty Katrina M Bird Amal Elhage Chloe Sligar Ronald Sluyter

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Humanized mouse models of graft-versus-host disease (GVHD), where human immune cells are injected into immune deficient mice, are well established and provide opportunities to investigate pathways involved in GVHD development. This chapter provides an overview of human immune cell isolation, injection of these cells into immune deficient mice, monitoring of mice for signs of GVHD, and assessment of human cell engraftment using flow cytometry. Further, this chapter focuses on the P2X7 signaling pathway involved in GVHD, and describes a strategy to block the P2X7 receptor and examine the effect of this on GVHD development.

Keyphrases

endothelial cells
mouse model
allogeneic hematopoietic stem cell transplantation
signaling pathway
flow cytometry
induced pluripotent stem cells
induced apoptosis
stem cells
metabolic syndrome
type diabetes
monoclonal antibody
adipose tissue
acute myeloid leukemia
cell cycle arrest
cell death