Nanoparticle-Based Discrimination of Single-Nucleotide Polymorphism in Long DNA Sequences.
María Sanromán-IglesiasCharles H LawrieLuis M Liz-MarzánMarek GrzeliczakPublished in: Bioconjugate chemistry (2017)
Circulating DNA (ctDNA) and specifically the detection cancer-associated mutations in liquid biopsies promises to revolutionize cancer detection. The main difficulty however is that the length of typical ctDNA fragments (∼150 bases) can form secondary structures potentially obscuring the mutated fragment from detection. We show that an assay based on gold nanoparticles (65 nm) stabilized with DNA (Au@DNA) can discriminate single nucleotide polymorphism in clinically relevant ssDNA sequences (70-140 bases). The preincubation step was crucial to this process, allowing sequential bridging of Au@DNA, so that single base mutation can be discriminated, down to 100 pM concentration.
Keyphrases
- circulating tumor
- cell free
- single molecule
- gold nanoparticles
- circulating tumor cells
- loop mediated isothermal amplification
- nucleic acid
- reduced graphene oxide
- high throughput
- real time pcr
- high resolution
- sensitive detection
- squamous cell carcinoma
- air pollution
- mass spectrometry
- ionic liquid
- risk assessment
- genetic diversity
- squamous cell
- wild type
- childhood cancer