Insights into Common Octopus ( Octopus vulgaris ) Ink Proteome and Bioactive Peptides Using Proteomic Approaches.
Md Abdus Shukur ImranMónica CarreraSara Pérez-PoloJaime PérezLorena BarrosSonia DiosCamino GestalPublished in: Marine drugs (2023)
The common octopus ( Octopus vulgaris ) is nowadays the most demanded cephalopod species for human consumption. This species was also postulated for aquaculture diversification to supply its increasing demand in the market worldwide, which only relies on continuously declining field captures. In addition, they serve as model species for biomedical and behavioral studies. Body parts of marine species are usually removed before reaching the final consumer as by-products in order to improve preservation, reduce shipping weight, and increase product quality. These by-products have recently attracted increasing attention due to the discovery of several relevant bioactive compounds. Particularly, the common octopus ink has been described as having antimicrobial and antioxidant properties, among others. In this study, the advanced proteomics discipline was applied to generate a common octopus reference proteome to screen potential bioactive peptides from fishing discards and by-products such as ink. A shotgun proteomics approach by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using an Orbitrap Elite instrument was used to create a reference dataset from octopus ink. A total of 1432 different peptides belonging to 361 non-redundant annotated proteins were identified. The final proteome compilation was investigated by integrated in silico studies, including gene ontology (GO) term enrichment, pathways, and network studies. Different immune functioning proteins involved in the innate immune system, such as ferritin, catalase, proteasome, Cu/Zn superoxide dismutase, calreticulin, disulfide isomerase, heat shock protein, etc., were found in ink protein networks. Additionally, the potential of bioactive peptides from octopus ink was addressed. These bioactive peptides can exert beneficial health properties such as antimicrobial, antioxidant, antihypertensive, and antitumoral properties and are therefore considered lead compounds for developing pharmacological, functional foods or nutraceuticals.
Keyphrases
- liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- ultra high performance liquid chromatography
- amino acid
- heat shock protein
- high performance liquid chromatography
- gas chromatography
- oxidative stress
- immune response
- simultaneous determination
- staphylococcus aureus
- high resolution mass spectrometry
- blood pressure
- high throughput
- genetic diversity
- label free
- healthcare
- endothelial cells
- mental health
- body mass index
- physical activity
- solid phase extraction
- gene expression
- molecular docking
- working memory
- preterm infants
- health information
- small molecule
- body composition
- case control
- binding protein
- anti inflammatory
- weight gain
- health insurance
- patient reported outcomes
- quality improvement
- hydrogen peroxide