Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification.
Zhian HuFujian XuGongwei SunSichun ZhangXinrong ZhangPublished in: Chemical communications (Cambridge, England) (2020)
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.