Evaluation of Matrix Complexity in Nontargeted Analysis of Small-Molecule Toxicants by Liquid Chromatography-High-Resolution Mass Spectrometry.
Shai DaganDana MarderNitzan TzananiEyal DrugHagit PrihedLilach Yishai-AviramPublished in: Analytical chemistry (2023)
Complex mixtures, characterized by high density of compounds, challenge trace detection and identification. This is further exacerbated in nontargeted analysis, where a compound of interest may be well hidden under thousands of matrix compounds. We studied the effect of matrix complexity on nontargeted detection (peak picking) by LC-MS/MS (Orbitrap) analysis. A series of ∼20 drugs, V-type chemical warfare agents and pesticides, simulating toxic unknowns, were spiked at various concentrations in several complex matrices including urine, rosemary leaves, and soil extracts. Orbitrap "TraceFinder" software was used to explore their peak intensities in relation to the matrix (peak location in an intensity-sorted list). Average practical detection limits of nontargets were determined. While detection among the first 10,000 peaks was achieved at 0.3-1 ng/mL levels in the extract, for the more realistic "top 1000" list, much higher concentrations were required, approaching 10-30 ng/mL. A negative power law functional dependence between the peak location in an intensity-sorted suspect list and the nontarget concentration is proposed. Controlled complexity was explored with a series of urine dilutions, resulting in an excellent correlation between the power law coefficient and dilution factor. The intensity distribution of matrix peaks was found to spread (unevenly) on a broad range, fitting well the Weibull distribution function with all matrices and extracts. The quantitative approach demonstrated here gives a measure of the actual capabilities and limitations of LC-MS in the analysis of nontargets in complex matrices. It may be used to estimate and compare the complexity of matrices and predict the typical detection limits of unknowns.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- mass spectrometry
- gas chromatography
- ultra high performance liquid chromatography
- tandem mass spectrometry
- loop mediated isothermal amplification
- small molecule
- label free
- high density
- real time pcr
- high resolution
- high intensity
- risk assessment
- magnetic resonance
- computed tomography
- simultaneous determination
- heavy metals
- sensitive detection