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Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil.

Michaela SlavíčkováMartina JanouškováAnna ŠimonováHana CahováMilada KambováHana ŠanderováLibor KrásnýMichal Hocek
Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2018)
Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5-ethynyluracil (UE ) with azides was used for turning off sequence-specific protein-DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI-HF. Finally, DNA template containing UE was used for in vitro transcription with E. coli RNA polymerase and the transcription was turned off by CuAAC with 3-azidopropane-1,2-diol or 3-azido-7-hydroxycoumarin.
Keyphrases
  • circulating tumor
  • cell free
  • single molecule
  • transcription factor
  • nucleic acid
  • escherichia coli
  • mass spectrometry
  • dna repair
  • protein protein
  • dna binding
  • oxide nanoparticles