In situ separation and visualization of isomeric auxin derivatives in Arabidopsis by ion mobility mass spectrometry imaging.
Chao ZhangKristýna BieleszováAsta ŽukauskaitėPavel HladíkJiří GrúzOndřej NovákKarel DoležalPublished in: Analytical and bioanalytical chemistry (2023)
In situ separation and visualization of synthetic and naturally occurring isomers from heterogeneous plant tissues, especially when they share similar molecular structures, are a challenging task. In this study, we combined the ion mobility separation with desorption electrospray ionization mass spectrometry imaging (DESI-IM-MSI) to achieve a direct separation and visualization of two synthetic auxin derivatives, auxinole and its structural isomer 4pTb-MeIAA, as well as endogenous auxins from Arabidopsis samples. Distinct distribution of these synthetic isomers and endogenous auxins in Arabidopsis primary roots and hypocotyls was achieved in the same imaging analysis from both individually treated and cotreated samples. We also observed putative metabolites of synthetic auxin derivatives, i.e. auxinole amino acid conjugates and hydrolysed 4pTb-MeIAA product - 4pTb-IAA, based on their unique drifting ion intensity patterns. Furthermore, DESI-IM-MSI-revealed abundance of endogenous auxins and synthetic isomers was validated by liquid chromatography-mass spectrometry (LC-MS). Our results demonstrate that DESI-IM-MSI could be used as a robust technique for detecting endogenous and exogenous isomers and provide a spatiotemporal evaluation of hormonomics profiles in plants.
Keyphrases
- liquid chromatography
- mass spectrometry
- high resolution
- high resolution mass spectrometry
- tandem mass spectrometry
- transcription factor
- simultaneous determination
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- solid phase extraction
- amino acid
- gene expression
- plant growth
- photodynamic therapy
- single cell
- wastewater treatment