Analysis of the antifungal potential of Macrocybe titans extract against Candida albicans .
Fernanda Cbn PereiraGabrielle C PeiterVivian Ems JustoGabrieli M HuffPollyanna Cv ConradoMauro Ap da SilvaPatrícia de Souza Bonfim-MendonçaTerezinha Inez Estivalet SvidzinskiFabio R RosadoAdriana FioriniPublished in: Future microbiology (2023)
Aim: To investigate the antifungal potential of Macrocybe titans extracts against Candida albicans . Material & methods: Extracts were obtained as aqueous (EfraMat-22 and EfraMat-45) and methanolic/ethyl acetate fractions. Results: Broth microdilution and disk diffusion assays showed that EfraMat-45 provided the best results in terms of minimum inhibitory concentration. Scanning electron microscopy analysis revealed morphological changes and slight damage on the surfaces of cells exposed to EfraMat-45 at the MIC. Fluorescence microscopy analysis of the yeasts showed cell elongation. EfraMat-45 presented high levels of phenolic compounds and flavonoids, high antioxidant activity and absence of in vitro cytotoxicity. Conclusion: The results indicated that the aqueous extract of M. titans is highly promising as an antifungal agent.
Keyphrases
- candida albicans
- electron microscopy
- biofilm formation
- oxidative stress
- ionic liquid
- single molecule
- single cell
- induced apoptosis
- high resolution
- high throughput
- cell cycle arrest
- cell therapy
- stem cells
- human health
- cell death
- staphylococcus aureus
- energy transfer
- bone marrow
- risk assessment
- cystic fibrosis
- pi k akt
- mass spectrometry
- pseudomonas aeruginosa