Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging.
Sílvia VieiraPaulina StrymeckaLuiza StanaszekJoana Silva-CorreiaKatarzyna DrelaMichał FiedorowiczIzabela Malysz-CymborskaMiroslaw JanowskiRui Luis ReisBarbara LukomskaPiotr WalczakJoaquim Miguel OliveiraPublished in: Bioengineering (Basel, Switzerland) (2023)
This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn 2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBP shi/shi /rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.
Keyphrases
- magnetic resonance imaging
- contrast enhanced
- hyaluronic acid
- drug delivery
- single cell
- computed tomography
- cell therapy
- room temperature
- tissue engineering
- magnetic resonance
- diffusion weighted imaging
- metal organic framework
- adipose tissue
- high throughput
- stem cells
- bone marrow
- acute respiratory distress syndrome
- skeletal muscle
- photodynamic therapy
- fluorescence imaging
- ultrasound guided
- quantum dots
- drug release
- solid state
- wild type