Detection of MSH2 Gene Methylation in Extramammary Paget's Disease by Methylation-Sensitive High-Resolution Melting Analysis.

Liu DongYingfeng ZhuLiting WuQiaoan ZhangFeng XuXinju ZhangXiao XuYiting TangGuoqiang RenZhihua Kang Ming Guan

Published in: Journal of oncology (2021)

MS-HRM standard curve can be used for the detection of the methylation level of MSH2 in EMPD tumor samples and other cancerous tissues potentially, which presents a promising candidate as a quantitative assay to analyze MSH2 promoter methylation in routine pathological procedure.

Keyphrases

high resolution
genome wide
dna methylation
gene expression
mass spectrometry
loop mediated isothermal amplification
multiple sclerosis
high throughput
label free
clinical practice
quantum dots