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Open-source recombinant monoclonal secondary nanobodies.

Helge Ewers
Published in: The Journal of cell biology (2018)
Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.
Keyphrases
  • high throughput
  • single cell
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  • south africa
  • multiple myeloma
  • stem cells
  • mesenchymal stem cells
  • bone marrow