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Tunable graphdiyne for DNA surface adsorption: affinities, displacement, and applications for fluorescence sensing.

Jing XiaoZhang LiuChunxiang LiJinglun WangHaowen HuangQingfeng YiKeqing DengXiaofang Li
Published in: Analytical and bioanalytical chemistry (2021)
Graphdiyne (GDY) adsorbed DNA probes have been used as a fluorescent sensing platform, but topics including DNA adsorption affinities, DNA probe displacement, and fluorescence quenching ability were rarely researched. Herein, the adsorption affinity of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) on a tremella-like GDY was tuned by modulating the surface chemistry of GDY. The fluorescence quenching ability of GDY with different oxidation degrees was compared. The nonspecific displacement of DNA probes on GDY was studied. Under the same concentrations, GDY with low oxidation degree exhibited stronger adsorption affinity and higher adsorption capacity to both ssDNA and dsDNA than highly oxidized GDY. DNA adsorbed on low-oxidized GDY was more resistant to displacement by other DNAs. Protein showed strong interaction with different GDY and could displace DNA probes on GDY. Based on these findings, an ideal GDY with proper oxidation degree, exhibiting high surface affinity for ssDNA and low affinity for dsDNA, was used as scavenger of redundant ssDNA fluorescent probe in an enzyme-assisted amplification system for sensitive ochratoxin (OTA) detection. This study has enhanced our fundamental understanding of DNA adsorption by GDY. It also provided a rational way to apply GDY for fluorescence sensing in a complicated system.
Keyphrases
  • single molecule
  • circulating tumor
  • living cells
  • nucleic acid
  • cell free
  • fluorescent probe
  • aqueous solution
  • circulating tumor cells
  • nitric oxide
  • binding protein
  • amino acid
  • electron transfer