A new polycyclic tetramate macrolactam from Allostreptomyces RD068384: stereochemistry and antifungal potential.
Marwa ElsbaeyYuki SamaruEngy ElekhnawyNaoya OkuYasuhiro IgarashiPublished in: The Journal of antibiotics (2024)
A new polycyclic tetramate macrolactam designated allostreptamide (1), together with four known congeners, were isolated from the culture extract of Allostreptomyces RD068384. The planar structure of the new compound was elucidated through interpretation of NMR and MS data. The absolute configuration was determined through ROESY and ECD analyses. The isolated compounds revealed antifungal potential against fourteen Candida albicans isolates with minimum inhibitory concentrations (MICs) ranging from 64 to 2048 µg ml -1 . Compound 3 showed antibiofilm action and considerably reduced the viability of five isolates (36%) in the formed biofilm. The qRT-PCR revealed that 3 downregulated the BCR1, PLB2, ALS1, and SAP5 biofilm related gene expression. Therefore, 3 could be a promising antifungal therapy for C. albicans infections.
Keyphrases
- candida albicans
- biofilm formation
- gene expression
- single cell
- acute lymphoblastic leukemia
- mass spectrometry
- magnetic resonance
- multiple sclerosis
- dna methylation
- oxidative stress
- high resolution
- genetic diversity
- electronic health record
- tyrosine kinase
- escherichia coli
- machine learning
- risk assessment
- artificial intelligence
- deep learning
- amyotrophic lateral sclerosis
- drug induced