Contamination of Animal Feed with Undeclared Tetracyclines-Confirmatory Analysis by Liquid Chromatography-Mass Spectrometry after Microbiological Plate Test.
Monika Przeniosło-SiwczyńskaEwelina PatyraAleksandra GrelikMaja Chyłek-PurchałaBeata KozakKrzysztof KwiatekPublished in: Molecules (Basel, Switzerland) (2020)
The presence of tetracycline (TC) antibiotics was determined in animal feed that had been previously screened with a microbiological plate test. Feed samples were screened by a microbiological plate test on a pH 6.0 culture medium seeded with Bacillus cereus ATCC 11778 able to pre-reveal the presence of tetracyclines. Subsequently, confirmation and quantification were performed using a validated HPLC method with mass spectrometric detection. In 2013-2018, 353 feed samples were analysed to detect antibacterial substances, of which 186 (52.7%) were suspected to contain tetracyclines. Forty-two out of 186 (22.6%) samples analysed by the chromatographic method contained undeclared tetracyclines, which were determined at concentrations from 0.3 to 49 mg kg-1. The most frequently identified contaminating tetracyclines were doxycycline and chlortetracycline.
Keyphrases
- mass spectrometry
- liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- high performance liquid chromatography
- ms ms
- high resolution mass spectrometry
- risk assessment
- gas chromatography
- solid phase extraction
- pulmonary embolism
- high resolution
- gene expression
- genome wide
- capillary electrophoresis
- health risk
- loop mediated isothermal amplification
- single cell
- real time pcr
- silver nanoparticles