A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay.
Juan Luis de la Fuente-JiménezCésar Iván Rodríguez-RivasIrma Beatriz Mitre-AguilarAndrea Torres-CopadoEric Alejandro García-LópezJosé Herrera-CelisMaría Goretti Arvizu-EspinosaMarco Antonio Garza-NavarroLuis Gerardo ArriagaJanet Ledesma GarcíaDomingo Ixcóatl García-GutiérrezAlejandro Zentella DehesaAshutosh SharmaGoldie OzaPublished in: International journal of molecular sciences (2023)
Zinc ferrite nanoparticles (ZFO NPs) are a promising magneto-crystalline platform for nanomedicine-based cancer theranostics. ZFO NPs synthesized using co-precipitation method are characterized using different techniques. UV-visible spectroscopy exhibits absorption peaks specific for ZFO. Raman spectroscopy identifies Raman active, infrared active, and silent vibrational modes while Fourier transforms infrared spectroscopic (FTIR) spectra display IR active modes that confirm the presence of ZFO. X-ray diffraction pattern (XRD) exhibits the crystalline planes of single-phase ZFO with a face-centered cubic structure that coincides with the selected area electron diffraction pattern (SAED). The average particle size according to high-resolution transmission electron microscopy (HR-TEM) is 5.6 nm. X-ray photoelectron spectroscopy (XPS) signals confirm the chemical states of Fe, Zn, and O. A superconducting quantum interference device (SQUID) displays the magnetic response of ZFO NPs, showing a magnetic moment of 45.5 emu/gm at 70 kOe. These ZFO NPs were then employed for comparative cytotoxicity evaluation using MTT, crystal violet, and LDH assays on breast adenocarcinoma epithelial cell (MCF-7), triple-negative breast cancer lines (MDA-MB 231), and human embryonic kidney cell lines (HEK-293). Flow cytometric analysis of all the three cell lines were performed in various concentrations of ZFO NPs for automated cell counting and sorting based on live cells, cells entering in early or late apoptotic phase, as well as in the necrotic phase. This analysis confirmed that ZFO NPs are more cytotoxic towards triple-negative breast cancer cells (MDA-MB-231) as compared to breast adenocarcinoma cells (MCF-7) and normal cell lines (HEK-293), thus corroborating that ZFO can be exploited for cancer therapeutics.
Keyphrases
- cell cycle arrest
- breast cancer cells
- high resolution
- electron microscopy
- oxide nanoparticles
- cell death
- induced apoptosis
- raman spectroscopy
- high throughput
- pi k akt
- endoplasmic reticulum stress
- papillary thyroid
- oxidative stress
- squamous cell carcinoma
- mass spectrometry
- single molecule
- room temperature
- deep learning
- risk assessment
- photodynamic therapy
- endothelial cells
- stem cells
- gene expression
- drug delivery
- machine learning
- locally advanced
- molecular dynamics
- density functional theory
- molecular dynamics simulations
- solid state
- genome wide
- dna methylation
- rectal cancer
- anti inflammatory
- high speed
- label free
- metal organic framework
- childhood cancer