Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels.
Anita BányaiEnikő FarkasHajnalka JankovicsInna SzékácsEszter Leelőssyné TóthFerenc VondervisztRobert HorvathMáté VargaPéter FürjesPublished in: Sensors (Basel, Switzerland) (2023)
The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli ( E. coli ), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae , and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.
Keyphrases
- induced apoptosis
- cell cycle arrest
- escherichia coli
- saccharomyces cerevisiae
- red blood cell
- cell death
- endoplasmic reticulum stress
- signaling pathway
- mesenchymal stem cells
- mass spectrometry
- staphylococcus aureus
- pi k akt
- molecular dynamics
- cell therapy
- circulating tumor cells
- living cells
- bone marrow
- atomic force microscopy
- single molecule
- high speed