StressMatic: A Novel Automated System to Induce Depressive- and Anxiety-like Phenotype in Rats.
Joana MacedoAntónio Mateus-PinheiroCátia AlvesFernando VelosoEduardo D GomesInês RibeiroJoana S CorreiaTiago Silveira-RosaNuno Dinis AlvesAna J RodriguesJoão M BessaNuno SousaJoão F OliveiraPatrícia PatrícioLuisa PintoPublished in: Cells (2023)
Major depressive disorder (MDD) is a multidimensional psychiatric disorder that is estimated to affect around 350 million people worldwide. Generating valid and effective animal models of depression is critical and has been challenging for neuroscience researchers. For preclinical studies, models based on stress exposure, such as unpredictable chronic mild stress (uCMS), are amongst the most reliable and used, despite presenting concerns related to the standardization of protocols and time consumption for operators. To overcome these issues, we developed an automated system to expose rodents to a standard uCMS protocol. Here, we compared manual (uCMS) and automated (auCMS) stress-exposure protocols. The data shows that the impact of the uCMS exposure by both methods was similar in terms of behavioral (cognition, mood, and anxiety) and physiological (cell proliferation and endocrine variations) measurements. Given the advantages of time and standardization, this automated method represents a step forward in this field of preclinical research.
Keyphrases
- major depressive disorder
- bipolar disorder
- stress induced
- sleep quality
- deep learning
- cell proliferation
- machine learning
- high throughput
- mental health
- big data
- cell cycle
- case report
- electronic health record
- heat stress
- artificial intelligence
- physical activity
- bone marrow
- pi k akt
- drug induced
- single cell
- data analysis