Detection of antibodies against domestic cat hepadnavirus using baculovirus-expressed core protein.
Paola FruciFederica Di ProfioAndrea PalombieriIvano MassirioGianvito LanaveGeorgia DiakoudiFrancesco PellegriniFulvio MarsilioVito MartellaBarbara Di MartinoPublished in: Transboundary and emerging diseases (2022)
A novel orthohepadnavirus (domestic cat hepadnavirus [DCH]) similar to human hepatitis B virus has been recently detected in serum and liver samples from domestic cats with chronic hepatitis and hepatocellular carcinoma. Molecular investigations by independent research groups around the world have revealed positivity rates ranging from 6.5% to 12.5% in blood samples and up to 14.0% in liver tissue. In this study, we screened an age-stratified collection of feline sera (n = 256) by using an antibody detection enzyme-linked immunosorbent assay based on the recombinant core antigen of DCH (DCHc). Specific antibodies (DCHc Abs) were detected with a prevalence of 25.0%. The DNA of DCH was detected in 35.9% (23/64) of seropositive cats and only in 1.0% (2/192) of seronegative animals. Based on the serological (IgG and IgM anti-DCHc) and virological status, the possible stages of DCH infection were predicted.
Keyphrases
- hepatitis b virus
- loop mediated isothermal amplification
- endothelial cells
- real time pcr
- single molecule
- label free
- liver failure
- cell free
- risk factors
- high throughput
- hiv infected
- single cell
- protein protein
- induced pluripotent stem cells
- small molecule
- binding protein
- quantum dots
- drug induced
- circulating tumor cells