Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles.
Dokyoung KimJinyoung KangTaejun WangHye Gun RyuJonathan M ZuidemaJinmyoung JooMuwoong KimYoungbuhm HuhJunyang JungKyo Han AhnKi Hean KimMichael J SailorPublished in: Advanced materials (Deerfield Beach, Fla.) (2017)
A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650-1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.
Keyphrases
- photodynamic therapy
- monte carlo
- living cells
- fluorescent probe
- fluorescence imaging
- gene expression
- visible light
- high resolution
- drug release
- cancer therapy
- light emitting
- energy transfer
- metal organic framework
- walled carbon nanotubes
- molecular dynamics
- drug delivery
- single molecule
- tissue engineering
- mass spectrometry