Transcriptome-wide N6-methyladenosine methylation landscape of coronary artery disease.
Keyong DengXiaotong NingXiaoxiao RenBin YangJianxin LiJie CaoJichun ChenXiangfeng LuShufeng ChenLaiyuan WangPublished in: Epigenomics (2021)
Aim: To reveal transcriptome-wide N6-methyladenosine (m6A) methylome of coronary artery disease (CAD). Materials & methods: The m6A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m6A methylome profiled by methylated RNA immunoprecipitation sequencing (MeRIP-seq) identified differentially methylated m6A sites within both mRNAs and lncRNAs between CAD and control group. Results: Bioinformatic analysis indicated that differentially methylated genes were involved in the pathogenesis of atherosclerosis. MeRIP-quantitative real-time PCR assay confirmed the reliability of MeRIP-seq data. Finally, the rat carotid artery balloon injury model was performed to confirm the role of m6A demethylase FTO in neointima formation. Conclusion: Our study provided a resource of differentially methylated m6A profile for uncovering m6A biological functions in the pathogenesis of CAD.
Keyphrases
- coronary artery disease
- genome wide
- single cell
- rna seq
- dna methylation
- high throughput
- percutaneous coronary intervention
- cardiovascular events
- coronary artery bypass grafting
- real time pcr
- cardiovascular disease
- gene expression
- oxidative stress
- heart failure
- genome wide analysis
- electronic health record
- left ventricular
- big data
- network analysis
- acute coronary syndrome
- nucleic acid
- smooth muscle
- transcatheter aortic valve replacement
- high throughput sequencing