A dual-color ESIPT-based probe for simultaneous detection of hydrogen sulfide and hydrazine.

Hydrogen sulfide (H 2 S) and hydrazine (N 2 H 4 ) are toxic compounds in environmental and living systems, and hydrogen sulfide is also an important signaling molecule. However, in the absence of dual-color probes capable of detecting both H 2 S and N 2 H 4 , the ability to monitor the crosstalk of these substances is restricted. Herein, we developed an ESIPT-based dual-response fluorescent probe (BDM-DNP) for H 2 S and N 2 H 4 detection via dually responsive sites. The BDM-DNP possessed absorbing strength in the detection of H 2 S and N 2 H 4 , with a large Stokes shift (156 nm for H 2 S and 108 nm for N 2 H 4 ), high selectivity and sensitivity, and good biocompatibility. Furthermore, BDM-DNP can be utilized for the detection of hydrogen sulfide and hydrazine in actual soil, and gaseous H 2 S and N 2 H 4 in environmental systems. Notably, BDM-DNP can detect H 2 S and N 2 H 4 in living cells for disease diagnosis and treatment evaluation.